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The BSP facility has experience isolating high molecular weight genomic DNA from a variety of sources including whole blood, buccal swabs and rinses, blood spots, and immortalized cells which has been shown to be reliably PCR amplified and genotyped using multiple platforms including TaqMan, Illumina GoldenGate, GWAS, and exome and whole genome sequencing. We additionally have extensive experience extracted RNA from a variety of sources including, PAXgene blood tubes, bronchcoscopy specimens, FFPE slides and cores, and dental plaque.

The BSP facility utilizes a specimen tracking system designed specifically to meet the immediate, inventory-style tracking needs of multiple health research projects and to be flexible and extendible for future needs.

We provide the following services:

  • Study set-up, including specimen collection. labeling, transport, tracking and reporting

 

  • Whole blood
  • Buffy coats
  • Blood spots
  • Buccal rinses
  • Buccal brushes
  • Saliva
  • Cell lines
  • Plasma
  • FFPE tissue
  • Other – inquire
  • Absorbance
  • Picogreen
  • Real-Time PCR
  • Normally four equal aliquots prepared unless study specific aliquoting needs are requested

 

  • PAXgene RNA blood tubes
  • FFPE slides or cores
  • Dental plaque
  • Other

 

  • RNA quality assessed by Agilent Tape Station
  • Serum
  • Plasma
  • Clot
  • Buffy Coat
  • Urine
  • Toenails
  • Other – inquire
  • Genotyping
  • Sequencing
  • Additional analyte analysis labs
  • Multi-institutional collaborators
  • Monitored and generator backed up -80 degree storage
  • Whole genome amplification
  • PCR amplification

This facility is currently equipped with  two high-throughput nucleic acid extraction robotic systems which isolates DNA/RNA from large-volume samples, two high throughput plate spectrophotometers, liquid handling robot, an Agilent Tape Station, Nanodrop, automated tube labeler, digital gel documentation system, real-time PCR machine, and the freezer capacity to store over 500,000 -80° C samples. Please see the Equipment page for more information.