Equipment

The BSP facility contains the following instruments:

Two Perkin-Elmer Chemagic MSM I for automated high throughput nucleic acid (NA) isolation systems, based on proprietary M-PVA magnetic bead technology. Provides ultra-clean NA preps from saliva, mouthrinses, small tissues, frozen blood and buffy coats: >96 samples per 8 hour period.

A Purigen Biosystem’s Ionic Purification System for automated purification of nucleic acids from small cell numbers and FFPE scrolls or slides requires less than 3 minutes of hands-on time per sample (lysis included). For DNA/RNA purification with this system, no chemical deparaffinization is required. In addition, nucleic acids are automatically separated and concentrated from sample lysate in 60 minutes.

Two high-throughput spectrophotometers for determining DNA quantitation and a plate-based luminometer

• Denovix DS-11FX Spectrophotomer/Fluorometer
• Caliper LabChipDs with CDrop software
• Molecular Devices SpectraMax plate spectrophotometer and luminometer

ABI 7300 real-time PCR machine

Applied Biosystems GeneAmp PCR System 9700

Scilogex SCI-100HCM-Pro Digital Thermal Mixer

Monitored freezer (-80° C) capacity for >400,000 cryovials

Perkin-Elmer Janus liquid handling and extraction robot

Agilent Tape Station

Micronic VX2 automated labeler